Hotspot

rpl32-trnL-UAG

SSC · 111684-112429 · 746 bp

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Pi 0.0742

MarkerSeek score 77.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 746
variable_sites: 59
pi: 0.0742
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	896-1401 bp	1.0	87.7
rpl32-trnL-UAG_p2	CTTTTTGACTGTCCGGTGGA	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1043-1548 bp	1.0	87.4
rpl32-trnL-UAG_p3	GGCCGAAAACCACATAGCTA	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1580-2416 bp	0.666667	69.3
rpl32-trnL-UAG_p4	GGCCGAAAACCACATAGCTA	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1727-2563 bp	0.666667	69.2
rpl32-trnL-UAG_p5	AGATTGGCCGAAAACCACAT	TGGCGAAACCTTGGGTAAAA	45.0	45.0	58.1	57.9	1732 bp	0.333333	52.2