Hotspot

trnI-CAU-ycf2

IRb · 85660-85727 · 68 bp

Back to Hetaeria

Pi 0.049

MarkerSeek score 70.00

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 68
variable_sites: 5
pi: 0.049
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TTGTTGCACCCTGTGAATCA	50.0	45.0	58.0	57.9	311-483 bp	1.0	80.8
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	AATCATGGTCCTTGCGGATC	50.0	50.0	58.0	58.0	797-969 bp	1.0	80.5
trnI-CAU-ycf2_p3	CACTTGACTGTCTACGGCTC	AATCATGGTCCTTGCGGATC	55.0	50.0	58.0	58.0	1370-1542 bp	1.0	80.4
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	CGAACCCCGGTTAGATTCAG	50.0	55.0	58.0	58.1	634-806 bp	1.0	80.4
trnI-CAU-ycf2_p5	CCACAACGATGCTGTCCATA	AATCATGGTCCTTGCGGATC	50.0	50.0	58.0	58.0	1417-1589 bp	1.0	80.3