Hotspot

psaI-ycf4

LSC · 58308-58690 · 383 bp

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Pi 0.0321

MarkerSeek score 69.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 18
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	AAAGGATAAAGGCCCAGCAA	45.0	45.0	57.9	57.4	518-523 bp	1.0	78.4
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	AAGGATAAAGGCCCAGCAAA	45.0	45.0	57.9	57.4	517-522 bp	1.0	78.4
psaI-ycf4_p3	GGCAATTGCAATGGCTTCTT	AGGATAAAGGCCCAGCAAAA	45.0	45.0	57.9	57.4	516-521 bp	1.0	78.4
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	AAAAGGATAAAGGCCCAGCA	45.0	45.0	57.9	57.4	519-524 bp	1.0	78.3
psaI-ycf4_p5	TAGTCTTTCCGGCAATTGCA	AGGATAAAGGCCCAGCAAAA	45.0	45.0	57.8	57.4	526-531 bp	1.0	77.6