Hotspot

petN-psbM

LSC · 27850-28402 · 553 bp

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Pi 0.0349

MarkerSeek score 68.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 28
pi: 0.0349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTATGGGGAAGGAGTGGG	AAAACAGTCAGCCAAAATAAT	55.0	28.6	57.5	52.2	603-630 bp	1.0	44.8
petN-psbM_p2	TAGTATGGGGAAGGAGTGGG	AAACAGTCAGCCAAAATAATT	55.0	28.6	57.5	52.2	602-629 bp	1.0	44.8
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AAAACAGTCAGCCAAAATAAT	50.0	28.6	58.2	52.2	660-687 bp	1.0	44.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	AAACAGTCAGCCAAAATAATT	50.0	28.6	58.2	52.2	659-686 bp	1.0	44.4
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AAAACAGTCAGCCAAAATAATT	50.0	27.3	58.2	53.1	660-687 bp	1.0	44.4