Hotspot

psbK-psbI

LSC · 7011-7452 · 442 bp

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Pi 0.0363

MarkerSeek score 69.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 22
pi: 0.0363
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	799-820 bp	1.0	86.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	800-821 bp	1.0	86.2
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TCTCCGACTCCCCTGATAAC	50.0	55.0	57.9	57.9	732-753 bp	1.0	85.9
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	CTCCGACTCCCCTGATAACT	50.0	55.0	57.9	57.9	731-752 bp	1.0	85.9
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	ACAAAAGAAGTACTGGCCCG	50.0	50.0	57.9	58.1	1023-1044 bp	1.0	84.7