Hotspot

trnK-UUU-rps16

LSC · 4223-4858 · 636 bp

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Pi 0.0456
MarkerSeek score 76.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
636
variable_sites
41
pi
0.0456
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 836-844 bp 1.0 83.8
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 835-843 bp 1.0 83.8
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1249-1260 bp 1.0 83.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1248-1259 bp 1.0 83.7
trnK-UUU-rps16_p5 AGGATCAGTCGCGGTCTTAT GCAAAGACAACGAATTCCGG 50.0 50.0 58.3 58.0 1334-1345 bp 1.0 82.4