Hotspot

matK-trnK-UUU

LSC · 3220-4185 · 966 bp

Back to Hetaeria

Pi 0.0293

MarkerSeek score 67.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 42
pi: 0.0293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCTTTGTAATTCTGTCATTTATAGACA	AACGGTAGAGTACTCGGCTT	25.9	50.0	55.2	58.2	1018-1043 bp	1.0	44.1
matK-trnK-UUU_p2	TCTTTGTAATTCTGTCATTTATAGACA	ACGGTAGAGTACTCGGCTTT	25.9	50.0	55.2	58.2	1017-1042 bp	1.0	44.1
matK-trnK-UUU_p3	CTTTGTAATTCTGTCATTTATAGACA	ACGGTAGAGTACTCGGCTTT	26.9	50.0	53.9	58.2	1016-1041 bp	1.0	44.1
matK-trnK-UUU_p4	TCTTTGTAATTCTGTCATTTATAGACA	ATCCGGGTTGCTAACTCAAC	25.9	50.0	55.2	57.9	1035-1060 bp	1.0	44.0
matK-trnK-UUU_p5	CTTTGTAATTCTGTCATTTATAGACA	ATCCGGGTTGCTAACTCAAC	26.9	50.0	53.9	57.9	1034-1059 bp	1.0	44.0