Hotspot

ccsA

SSC · 116840-117805 · 966 bp

Pi 0.0069

MarkerSeek score 49.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 10
pi: 0.0069
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TGGGCCACGAGAACTATTTG	50.0	50.0	57.9	57.9	1476-1493 bp	1.0	79.5
ccsA_p2	TAGACACGCTGCTCTTAGGA	GGGCCACGAGAACTATTTGT	50.0	50.0	57.9	57.9	1475-1492 bp	1.0	79.5
ccsA_p3	TAGACACGCTGCTCTTAGGA	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	1690-1707 bp	0.666667	66.2
ccsA_p4	AAATTGGTAGACACGCTGCT	TTGCATTGCCAGGAATGAGT	45.0	45.0	57.8	58.1	1697-1714 bp	0.666667	65.7
ccsA_p5	AGGAGCCCTTATTCATCCGT	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.2	58.1	1804-1821 bp	0.666667	65.6