Hotspot

ccsA-ndhD

SSC · 117806-118044 · 239 bp

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Pi 0.0335
MarkerSeek score 61.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
239
variable_sites
12
pi
0.0335
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGGCACTTGTAGCTTCTACA ACAAAGTACAAGCTATCCTATCT 50.0 34.8 57.6 54.6 393-410 bp 1.0 42.4
ccsA-ndhD_p2 TCGGCACTTGTAGCTTCTAC ACAAAGTACAAGCTATCCTATCT 50.0 34.8 57.4 54.6 394-411 bp 1.0 42.4
ccsA-ndhD_p3 ATTCGGCACTTGTAGCTTCT ACAAAGTACAAGCTATCCTATCT 45.0 34.8 57.2 54.6 396-413 bp 1.0 42.4
ccsA-ndhD_p4 CGGCACTTGTAGCTTCTACA ACAAAGTACAAGCTATCCTATC 50.0 36.4 57.6 53.1 393-410 bp 1.0 42.4
ccsA-ndhD_p5 CGAATTCGGCACTTGTAGCT ACAAAGTACAAGCTATCCTATCT 50.0 34.8 58.6 54.6 399-416 bp 1.0 42.3