Hotspot

rpl32-trnL-UAG

SSC · 115969-116682 · 714 bp

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Pi 0.0281
MarkerSeek score 64.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
714
variable_sites
29
pi
0.0281
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TAGACACGGCACTATGAGCT CCCTTTCCGATGAATCACGT 50.0 50.0 58.2 58.0 1442-1469 bp 1.0 81.7
rpl32-trnL-UAG_p2 TAGACACGGCACTATGAGCT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1194-1221 bp 1.0 81.6
rpl32-trnL-UAG_p3 TAGACACGGCACTATGAGCT TGGCGAAACCTTGGGTAAAA 50.0 45.0 58.2 57.9 1652-1679 bp 1.0 81.0
rpl32-trnL-UAG_p4 TAGACACGGCACTATGAGCT GGGTAAAAATGGCACTTGGC 50.0 50.0 58.2 57.9 1640-1667 bp 1.0 80.8
rpl32-trnL-UAG_p5 TAGACACGGCACTATGAGCT ACTGAAAATAACGACCCGCA 50.0 45.0 58.2 57.8 1785-1812 bp 1.0 79.9