Hotspot

trnR-ACG-trnN-GUU

IRb · 110661-111264 · 604 bp

Back to Hessea

Pi 0.00442
MarkerSeek score 61.60
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
604
variable_sites
4
pi
0.00442
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 745-746 bp 1.0 78.7
trnR-ACG-trnN-GUU_p2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.7 58.0 782-783 bp 1.0 78.1
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 696-697 bp 1.0 77.5
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG CTGGTCGTAGGTTCGAATCC 55.0 55.0 57.8 57.8 697-698 bp 1.0 77.1
trnR-ACG-trnN-GUU_p5 GAACAGATCCAGTGGAGACG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.7 58.2 733-734 bp 1.0 76.9