Hotspot

psbH-petB

LSC · 76743-76858 · 116 bp

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Pi 0.0187
MarkerSeek score 65.60
Primer available yes
Label psbH-petB

Feature Metadata

label_name
psbH-petB
feature_type
igs
region
LSC
length_bp
116
variable_sites
3
pi
0.0187
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.92
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH-petB_p1 CTGGGGGACTACACCACTTA ATGACAGGGCTCTTCTCTGT 55.0 50.0 58.1 58.1 281-290 bp 1.0 82.6
psbH-petB_p2 CGCAATGGCTTTATTTGCGA ATGACAGGGCTCTTCTCTGT 45.0 50.0 58.1 58.1 254-263 bp 1.0 82.6
psbH-petB_p3 CTGGGGGACTACACCACTTA ATCCGACGGGGTAGAATCAT 55.0 50.0 58.1 58.0 311-320 bp 1.0 82.5
psbH-petB_p4 CGCAATGGCTTTATTTGCGA ATCCGACGGGGTAGAATCAT 45.0 50.0 58.1 58.0 284-293 bp 1.0 82.5
psbH-petB_p5 TCGCAATGGCTTTATTTGCG ATCCGACGGGGTAGAATCAT 45.0 50.0 58.1 58.0 285-294 bp 1.0 82.5