Hotspot

ycf4-cemA

LSC · 62390-63204 · 815 bp

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Pi 0.0123
MarkerSeek score 57.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
815
variable_sites
15
pi
0.0123
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA GGAGGGAAGTCGATGCTTTC 45.0 55.0 57.9 58.3 872-881 bp 1.0 78.6
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG GGAGGGAAGTCGATGCTTTC 50.0 55.0 58.1 58.3 874-883 bp 1.0 78.3
ycf4-cemA_p3 GGAACCATTCCCTTGACTCG GGAGGGAAGTCGATGCTTTC 55.0 55.0 58.3 58.3 954-963 bp 1.0 77.0
ycf4-cemA_p4 TTTCTTGCGCGTACCAATTG GGAGGGAAGTCGATGCTTTC 45.0 55.0 57.7 58.3 877-886 bp 1.0 76.7
ycf4-cemA_p5 CCTATTTCTTGCGCGTACCA GGAGGGAAGTCGATGCTTTC 50.0 55.0 58.4 58.3 881-890 bp 1.0 76.4