Hotspot

trnR-UCU-atpA

LSC · 10435-10552 · 118 bp

Back to Hessea

Pi 0.0282
MarkerSeek score 70.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
118
variable_sites
5
pi
0.0282
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AAAGAAAGGGGGAGAATGCG TTCGACCAAGACATTCACCG 50.0 50.0 58.2 58.2 327 bp 1.0 78.3
trnR-UCU-atpA_p2 AAAGGGGGAGAATGCGAAAG TTCGACCAAGACATTCACCG 50.0 50.0 58.2 58.2 323 bp 1.0 78.3
trnR-UCU-atpA_p3 GAAAGGGGGAGAATGCGAAA TTCGACCAAGACATTCACCG 50.0 50.0 58.2 58.2 324 bp 1.0 78.2
trnR-UCU-atpA_p4 GGGGAGAATGCGAAAGAAGA TTCGACCAAGACATTCACCG 50.0 50.0 57.6 58.2 319 bp 1.0 76.4
trnR-UCU-atpA_p5 AAAGGGGGAGAATGCGAAAG CCAAGACATTCACCGAGCAA 50.0 50.0 58.2 58.5 318 bp 1.0 76.2