Hotspot

trnS-GCU-trnG-GCC

LSC · 8314-9446 · 1133 bp

Back to Hessea

Pi 0.0124
MarkerSeek score 58.90
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1133
variable_sites
21
pi
0.0124
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGTCGCTTTAGTCCA CGGACGAATCACACTTTTACC 50.0 47.6 58.0 57.3 1198-1221 bp 1.0 68.1
trnS-GCU-trnG-GCC_p2 AATCCGTCGCTTTAGTCCAC CGGACGAATCACACTTTTACC 50.0 47.6 58.0 57.3 1197-1220 bp 1.0 68.1
trnS-GCU-trnG-GCC_p3 CGGAGAGAGAGGGATTCGAA CGGACGAATCACACTTTTACC 55.0 47.6 58.3 57.3 1251-1274 bp 1.0 65.6
trnS-GCU-trnG-GCC_p4 AGCAATCCGTCGCTTTAGTC CGGACGAATCACACTTTTACC 50.0 47.6 58.4 57.3 1200-1223 bp 1.0 65.3
trnS-GCU-trnG-GCC_p5 CGTCGCTTTAGTCCACTCAG CGGACGAATCACACTTTTACC 55.0 47.6 58.4 57.3 1193-1216 bp 1.0 65.1