Hotspot

psbK-psbI

LSC · 7829-7993 · 165 bp

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Pi 0.0242
MarkerSeek score 62.30
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
165
variable_sites
6
pi
0.0242
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG ATTCCTCACGTCCGGGATTA 50.0 50.0 57.9 58.2 304 bp 1.0 79.4
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG TATTCCTCACGTCCGGGATT 50.0 50.0 57.9 58.2 305 bp 1.0 79.4
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TTATTCCTCACGTCCGGGAT 50.0 50.0 57.9 58.2 306 bp 1.0 79.4
psbK-psbI_p4 GTTTGGCAAGCTGCTGTAAG GGCCCGGATCGTTAGATAAG 50.0 55.0 57.9 57.6 283 bp 1.0 78.3
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG TTTTATTCCTCACGTCCGGG 50.0 50.0 57.9 57.6 308 bp 1.0 78.0