Hotspot

rps16-trnQ-UUG

LSC · 5759-7233 · 1475 bp

Back to Hessea

Pi 0.0129
MarkerSeek score 63.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1475
variable_sites
28
pi
0.0129
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCGAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1571-1573 bp 1.0 80.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1548-1550 bp 1.0 80.1
rps16-trnQ-UUG_p3 ATCCACAGCGAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1575-1577 bp 1.0 79.8
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1559-1561 bp 1.0 79.6
rps16-trnQ-UUG_p5 CCACAGCGAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1569-1571 bp 1.0 79.1