Hotspot

psbA-trnK-UUU

LSC · 1150-1387 · 238 bp

Pi 0.0252

MarkerSeek score 54.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 9
pi: 0.0252
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	CCCAGAAACAGGTTCACGAA	TTGCGAAGATTAGGTTCGGG	50.0	50.0	58.0	58.0	915-921 bp	1.0	80.9
psbA-trnK-UUU_p2	CCCAGAAACAGGTTCACGAA	CCATGTGGTGGTTGAATCCA	50.0	50.0	58.0	58.1	650-656 bp	1.0	80.9
psbA-trnK-UUU_p3	TAGCGGACTCGTTTTCAGTG	TTGCGAAGATTAGGTTCGGG	50.0	50.0	57.9	58.0	1408-1414 bp	1.0	80.3
psbA-trnK-UUU_p4	ATGCAACAGCAATCCAAGGA	TTGCGAAGATTAGGTTCGGG	45.0	50.0	58.1	58.0	1147-1153 bp	1.0	80.3
psbA-trnK-UUU_p5	GGTTACCAAGGAACCATGCA	TTGCGAAGATTAGGTTCGGG	50.0	50.0	58.1	58.0	1368-1374 bp	1.0	80.1