Hotspot

rpoC2

LSC · 17576-21703 · 4128 bp

Back to Hesperomeles

Pi 0.000906

MarkerSeek score 54.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4128
variable_sites: 10
pi: 0.000906
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTTGTTGAATCCGTGCCTTG	GCCCGCTCTTCCTTTATTGA	50.0	50.0	57.9	58.0	199-205 bp	1.0	82.5
rpoC2_p2	TTGAATCCGTGCCTTGGAAT	GCCCGCTCTTCCTTTATTGA	45.0	50.0	57.8	58.0	195-201 bp	1.0	81.2
rpoC2_p3	GTTGTTGAATCCGTGCCTTG	TTCTTCTATCGAGGAGGCCC	50.0	55.0	57.9	58.3	215-221 bp	1.0	80.2
rpoC2_p4	TTGAATCCGTGCCTTGGAAT	TTCTTCTATCGAGGAGGCCC	45.0	55.0	57.8	58.3	211-217 bp	1.0	79.0
rpoC2_p5	AATGGTTGTTGAATCCGTGC	GCCCGCTCTTCCTTTATTGA	45.0	50.0	57.3	58.0	203-209 bp	1.0	77.0