Hotspot

rpl32-trnL-UAG

SSC · 118026-119357 · 1332 bp

Back to Hesperomeles

Pi 0.00255

MarkerSeek score 60.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1332
variable_sites: 8
pi: 0.00255
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1444-1507 bp	1.0	79.0
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1445-1508 bp	1.0	79.0
rpl32-trnL-UAG_p3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1446-1509 bp	1.0	78.6
rpl32-trnL-UAG_p4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1447-1510 bp	1.0	78.6
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1454-1517 bp	1.0	78.0