Hotspot

ndhC-trnV-UAC

LSC · 54218-54922 · 705 bp

Back to Hesperomeles

Pi 0.0128

MarkerSeek score 71.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 21
pi: 0.0128
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	845-997 bp	1.0	83.4
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	781-933 bp	1.0	83.2
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	848-1000 bp	1.0	82.2
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	847-999 bp	1.0	82.2
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	58.3	58.2	862-1014 bp	1.0	81.8