Hotspot

psbM-trnD-GUC

LSC · 31518-32758 · 1241 bp

Back to Hesperomeles

Pi 0.000967
MarkerSeek score 57.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1241
variable_sites
3
pi
0.000967
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.6 58.5 1397-1437 bp 1.0 74.0
psbM-trnD-GUC_p2 AATCACCTTGGCTGACTGTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1399-1439 bp 1.0 72.2
psbM-trnD-GUC_p3 ATCACCTTGGCTGACTGTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1398-1438 bp 1.0 72.2
psbM-trnD-GUC_p4 TCACCTTGGCTGACTGTTTT GTTCAATTGGTCAGAGCACC 45.0 50.0 57.6 57.0 1398-1438 bp 1.0 69.8
psbM-trnD-GUC_p5 ATCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 42.9 50.0 58.1 58.5 1398-1438 bp 1.0 69.1