Hotspot

rps16-trnQ-UUG

LSC · 6316-7103 · 788 bp

Back to Hesperomeles

Pi 0.00389

MarkerSeek score 55.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 7
pi: 0.00389
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	872-956 bp	1.0	79.1
rps16-trnQ-UUG_p2	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	882-966 bp	1.0	77.4
rps16-trnQ-UUG_p3	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	849-933 bp	1.0	77.3
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	883-967 bp	1.0	77.3
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	934-1018 bp	1.0	76.8