Hotspot

ycf3

Genome · 85520-87436 · 1917 bp

Back to Hesperocyparis

Pi 0.00113

MarkerSeek score 58.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: Genome
length_bp: 1917
variable_sites: 4
pi: 0.00113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TTATGCTATTGTGCGTTGGC	AGCTAATTCGTTCGAATAGCA	45.0	38.1	57.2	55.5	2062-2063 bp	1.0	45.6
ycf3_p2	TTATGCTATTGTGCGTTGGC	GAAGCTAATTCGTTCGAATAGCA	45.0	39.1	57.2	57.4	2064-2065 bp	1.0	44.3
ycf3_p3	TTATGCTATTGTGCGTTGGC	AAGCTAATTCGTTCGAATAGCA	45.0	36.4	57.2	56.3	2063-2064 bp	1.0	43.6
ycf3_p4	TTATGCTATTGTGCGTTGGC	AGAGAAGCTAATTCGTTCGA	45.0	40.0	57.2	54.3	2067-2068 bp	1.0	43.5
ycf3_p5	TTATGCTATTGTGCGTTGGC	AGAAGCTAATTCGTTCGAATAGC	45.0	39.1	57.2	57.1	2065-2066 bp	1.0	42.3