Hotspot

ycf2

Genome · 48248-55684 · 7437 bp

Back to Hesperocyparis

Pi 0.000336

MarkerSeek score 55.00

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7437
variable_sites: 5
pi: 0.000336
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TACTCGGGTTCATAGCGAGA	AGAAATGCCGATCTTCGCTG	50.0	50.0	57.7	58.7	261 bp	1.0	72.0
ycf2_p2	TACTCGGGTTCATAGCGAGA	AGCAGAAATGCCGATCTTCG	50.0	50.0	57.7	58.7	264 bp	1.0	72.0
ycf2_p3	TACTCGGGTTCATAGCGAGA	CAGAAATGCCGATCTTCGCT	50.0	50.0	57.7	58.7	262 bp	1.0	72.0
ycf2_p4	GGGTTCATAGCGAGACAGAA	AGAAATGCCGATCTTCGCTG	50.0	50.0	57.0	58.7	256 bp	1.0	67.0
ycf2_p5	GGGTTCATAGCGAGACAGAA	CAGAAATGCCGATCTTCGCT	50.0	50.0	57.0	58.7	257 bp	1.0	67.0