Hotspot

chlB-trnK-UUU

Genome · 120947-122050 · 1104 bp

Back to Hesperocyparis

Pi 0.000453

MarkerSeek score 59.60

Primer available yes

Label chlB-trnK-UUU

Feature Metadata

label_name: chlB-trnK-UUU
feature_type: igs
region: Genome
length_bp: 1104
variable_sites: 1
pi: 0.000453
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnK-UUU_p1	TTGCACGACAAAAGGGGATT	CCGAGTACTCTGCCATTGAG	45.0	55.0	58.3	57.8	1204-1208 bp	1.0	76.3
chlB-trnK-UUU_p2	TTTGCACGACAAAAGGGGAT	CCGAGTACTCTGCCATTGAG	45.0	55.0	58.3	57.8	1205-1209 bp	1.0	76.3
chlB-trnK-UUU_p3	TTGCACGACAAAAGGGGATT	TGCCATTGAGTTAGCAACCC	45.0	50.0	58.3	58.5	1194-1198 bp	1.0	74.3
chlB-trnK-UUU_p4	TTTGCACGACAAAAGGGGAT	TGCCATTGAGTTAGCAACCC	45.0	50.0	58.3	58.5	1195-1199 bp	1.0	74.3
chlB-trnK-UUU_p5	TCCTCGTTTTGCCAGAGAAA	CCGAGTACTCTGCCATTGAG	45.0	55.0	57.4	57.8	1248-1252 bp	1.0	73.6