Hotspot

psbI-psbK

Genome · 118081-118685 · 605 bp

Back to Hesperocyparis

Pi 0.000826

MarkerSeek score 50.10

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: Genome
length_bp: 605
variable_sites: 1
pi: 0.000826
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	TCTTTACGCCCAGGATTTCG	CAAGCTGCCGTAAGTTTTCG	50.0	50.0	58.0	58.0	735-736 bp	1.0	79.7
psbI-psbK_p2	TCTTTACGCCCAGGATTTCG	AGCTGCCGTAAGTTTTCGAT	50.0	45.0	58.0	57.9	733-734 bp	1.0	79.3
psbI-psbK_p3	TCTTTACGCCCAGGATTTCG	TTTGCCAAATTGCCTGAAGC	50.0	45.0	58.0	58.1	831-832 bp	1.0	79.1
psbI-psbK_p4	TCTTTACGCCCAGGATTTCG	AAGCTGCCGTAAGTTTTCGA	50.0	45.0	58.0	58.1	734-735 bp	1.0	79.1
psbI-psbK_p5	CTTTACGCCCAGGATTTCGT	CAAGCTGCCGTAAGTTTTCG	50.0	50.0	58.3	58.0	734-735 bp	1.0	77.7