Hotspot

rrn16-trnI-GAU

Genome · 63065-64030 · 966 bp

Back to Hesperocyparis

Pi 0.00466

MarkerSeek score 61.90

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: Genome
length_bp: 966
variable_sites: 8
pi: 0.00466
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	AGTGACTGGAGTGAAGTCGT	ACCACTGAGCTAATAGCCCA	50.0	50.0	58.3	58.1	1089-1101 bp	1.0	77.2
rrn16-trnI-GAU_p2	AAGTCGTAACAAGGTAGCCG	ACCACTGAGCTAATAGCCCA	50.0	50.0	57.6	58.1	1076-1088 bp	1.0	76.8
rrn16-trnI-GAU_p3	AACAAGGTAGCCGTACTGGA	ACCACTGAGCTAATAGCCCA	50.0	50.0	58.4	58.1	1069-1081 bp	1.0	76.7
rrn16-trnI-GAU_p4	GGCTAGTGACTGGAGTGAAG	ACCACTGAGCTAATAGCCCA	55.0	50.0	57.3	58.1	1093-1105 bp	1.0	74.3
rrn16-trnI-GAU_p5	TGCCATGCTCGAAGTCATTA	ACCACTGAGCTAATAGCCCA	45.0	50.0	57.3	58.1	1146-1158 bp	1.0	74.1