Hotspot

ycf1-trnL-CAA

Genome · 47070-47966 · 897 bp

Back to Hesperocyparis

Pi 0.00279

MarkerSeek score 60.70

Primer available yes

Label ycf1-trnL-CAA

Feature Metadata

label_name: ycf1-trnL-CAA
feature_type: igs
region: Genome
length_bp: 897
variable_sites: 5
pi: 0.00279
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnL-CAA_p1	CGGACCAATTGGCAATGTTG	CATTTTGAGTGTCGCGTGTC	50.0	50.0	58.3	58.1	1033-1250 bp	1.0	77.5
ycf1-trnL-CAA_p2	CGGACCAATTGGCAATGTTG	GACATTTTGAGTGTCGCGTG	50.0	50.0	58.3	58.1	1035-1252 bp	1.0	77.5
ycf1-trnL-CAA_p3	CGGACCAATTGGCAATGTTG	ATTTTGAGTGTCGCGTGTCT	50.0	45.0	58.3	58.1	1032-1249 bp	1.0	77.0
ycf1-trnL-CAA_p4	CGGACCAATTGGCAATGTTG	TTTGAGTGTCGCGTGTCTAC	50.0	50.0	58.3	58.2	1030-1247 bp	1.0	76.1
ycf1-trnL-CAA_p5	AAATACATTTGGGCCGGACC	CATTTTGAGTGTCGCGTGTC	50.0	50.0	58.5	58.1	1047-1264 bp	1.0	75.7