Hotspot

rps12-clpP

Genome · 36519-37682 · 1164 bp

Back to Hesperocyparis

Pi 0.00258

MarkerSeek score 63.00

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 1164
variable_sites: 6
pi: 0.00258
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GCATACTCCCTTCCGTTGAG	GCAGGTTGTTTTTCTAGCGC	55.0	50.0	58.1	58.3	1326-1343 bp	1.0	78.8
rps12-clpP_p2	TTTCTTATCGGCTGTCTCGC	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1274-1291 bp	1.0	78.7
rps12-clpP_p3	CTTATCGGCTGTCTCGCATT	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1271-1288 bp	1.0	78.2
rps12-clpP_p4	TATCGGCTGTCTCGCATTTC	GCAGGTTGTTTTTCTAGCGC	50.0	50.0	58.1	58.3	1269-1286 bp	1.0	78.2
rps12-clpP_p5	TTATCGGCTGTCTCGCATTT	GCAGGTTGTTTTTCTAGCGC	45.0	50.0	57.7	58.3	1270-1287 bp	1.0	76.9