Hotspot

rps16

LSC · 5041-6183 · 1143 bp

Back to Hesperis

Pi 0.0175

MarkerSeek score 63.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1143
variable_sites: 33
pi: 0.0175
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GATGGAAGCGAAAGAGGAGG	ACGATATACTGACTGAACTATGACT	55.0	36.0	58.1	57.0	1306-1312 bp	1.0	42.3
rps16_p2	GATGGAAGCGAAAGAGGAGG	ACTGACTGAACTATGACTATTCATGA	55.0	34.6	58.1	57.7	1299-1305 bp	1.0	42.3
rps16_p3	GATGGAAGCGAAAGAGGAGG	ACGATATACTGACTGAACTATGAC	55.0	37.5	58.1	55.7	1306-1312 bp	1.0	42.3
rps16_p4	ATGGAAGCGAAAGAGGAGGA	ACGATATACTGACTGAACTATGACT	50.0	36.0	58.4	57.0	1305-1311 bp	1.0	42.3
rps16_p5	GATGGAAGCGAAAGAGGAGG	ACTGACTGAACTATGACTATTCA	55.0	34.8	58.1	54.6	1299-1305 bp	1.0	42.3