Hotspot

rpl32-trnL-UAG

SSC · 113445-114075 · 631 bp

Back to Hesperis

Pi 0.0625

MarkerSeek score 88.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 631
variable_sites: 60
pi: 0.0625
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	625-1074 bp	1.0	74.8
rpl32-trnL-UAG_p2	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	618-1067 bp	1.0	74.4
rpl32-trnL-UAG_p3	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	624-1073 bp	1.0	74.2
rpl32-trnL-UAG_p4	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	623-1072 bp	1.0	74.2
rpl32-trnL-UAG_p5	GGAAGGGATATTGGACATCGT	GAGCAGCGTGTCTACCAATT	47.6	50.0	57.2	58.3	619-1068 bp	1.0	73.6