Hotspot

accD-psaI

LSC · 58289-58932 · 644 bp

Back to Hesperis

Pi 0.0119

MarkerSeek score 61.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 12
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACGTAAGGGCTTGTTGGATG	AAAGAAGCCATTGCAAGTGC	50.0	45.0	58.2	57.8	827-858 bp	1.0	78.6
accD-psaI_p2	ACGTAAGGGCTTGTTGGATG	CCGGAAAGACTAGGCCTACT	50.0	55.0	58.2	58.2	808-839 bp	1.0	78.2
accD-psaI_p3	GGGCTTGTTGGATGCAATTG	AAAGAAGCCATTGCAAGTGC	50.0	45.0	58.3	57.8	821-852 bp	1.0	77.9
accD-psaI_p4	ACGTAAGGGCTTGTTGGATG	CAAGTGCCGGAAAGACTAGG	50.0	55.0	58.2	58.3	814-845 bp	1.0	77.7
accD-psaI_p5	TAAGGGCTTGTTGGATGCAA	AAAGAAGCCATTGCAAGTGC	45.0	45.0	57.7	57.8	824-855 bp	1.0	77.7