Hotspot

rbcL-accD

LSC · 56141-56833 · 693 bp

Back to Hesperis

Pi 0.0413

MarkerSeek score 75.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 52
pi: 0.0413
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCAATGCACGCTGTTATTG	ATTGCTTTACCTAGCTCGCC	45.0	50.0	58.0	58.0	1315-1336 bp	1.0	84.2
rbcL-accD_p2	TCATGGTATGCACTTCCGTG	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1427-1448 bp	1.0	83.9
rbcL-accD_p3	TGCAATGCACGCTGTTATTG	ATTCCGAATGTCCTTGGAGC	45.0	50.0	58.0	58.0	1460-1481 bp	1.0	83.8
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	ATTGCTTTACCTAGCTCGCC	50.0	50.0	58.0	58.0	1482-1503 bp	1.0	83.7
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1627-1648 bp	1.0	83.4