Hotspot

ndhK-ndhC

LSC · 49634-49697 · 64 bp

Back to Hesperis

Pi 0.0317

MarkerSeek score 65.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 3
pi: 0.0317
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GTTGTTCGATCAAGTACGGGA	TGTACTGGGGGTATCTGCTT	47.6	50.0	58.0	57.7	200-205 bp	1.0	72.6
ndhK-ndhC_p2	GTTGTTCGATCAAGTACGGGA	GAGTTTCGATGTACTGGGGG	47.6	55.0	58.0	57.7	209-214 bp	1.0	72.1
ndhK-ndhC_p3	GTTGTTCGATCAAGTACGGGA	AGTTTCGATGTACTGGGGGT	47.6	50.0	58.0	58.3	208-213 bp	1.0	71.9
ndhK-ndhC_p4	GTTGTTCGATCAAGTACGGGA	ATGTACTGGGGGTATCTGCT	47.6	50.0	58.0	57.5	201-206 bp	1.0	70.8
ndhK-ndhC_p5	TGTTGTTCGATCAAGTACGGG	TGTACTGGGGGTATCTGCTT	47.6	50.0	58.3	57.7	201-206 bp	1.0	70.5