Hotspot

trnF-GAA-ndhJ

LSC · 47950-48375 · 426 bp

Back to Hesperis

Pi 0.0282

MarkerSeek score 61.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 21
pi: 0.0282
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	519-566 bp	1.0	86.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	492-539 bp	1.0	86.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	491-538 bp	1.0	86.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	50.0	50.0	58.1	57.9	516-563 bp	1.0	86.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	50.0	50.0	58.1	58.2	526-573 bp	1.0	84.8