Hotspot

trnL-UAA-trnF-GAA

LSC · 47546-47876 · 331 bp

Back to Hesperis

Pi 0.126

MarkerSeek score 76.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 44
pi: 0.126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	421-649 bp	1.0	87.1
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	418-646 bp	1.0	83.9
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	410-638 bp	1.0	83.3
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	393-621 bp	1.0	83.1
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	397-625 bp	1.0	83.0