Hotspot

lhbA-trnG-UCC

LSC · 36010-36479 · 470 bp

Back to Hesperis

Pi 0.0142

MarkerSeek score 64.40

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 11
pi: 0.0142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	590-620 bp	1.0	82.2
lhbA-trnG-UCC_p2	CCGTTGTATTTGCGTCTCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	606-636 bp	1.0	81.7
lhbA-trnG-UCC_p3	TTTGCGTCTCCTGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	598-628 bp	1.0	80.2
lhbA-trnG-UCC_p4	ATTTGCGTCTCCTGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	599-629 bp	1.0	80.0
lhbA-trnG-UCC_p5	CGTTGTATTTGCGTCTCCTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	605-635 bp	1.0	77.6