Hotspot

rpoB-trnC-GCA

LSC · 26394-27461 · 1068 bp

Back to Hesperis

Pi 0.0401

MarkerSeek score 70.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 65
pi: 0.0401
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTGGATCCCCACCTACACA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1843-1867 bp	1.0	82.6
rpoB-trnC-GCA_p2	ATTGGATCCCCACCTACACA	AGCGTCTGTTCCTACGTTTG	50.0	50.0	58.0	58.2	1902-1917 bp	0.5	58.5
rpoB-trnC-GCA_p3	ATTGGATCCCCACCTACACA	CAAGCGTCTGTTCCTACGTT	50.0	50.0	58.0	58.2	1904-1919 bp	0.5	58.5
rpoB-trnC-GCA_p4	ATTGGATCCCCACCTACACA	ACGGATTTCCCTCCACTACT	50.0	50.0	58.0	57.8	2015-2030 bp	0.5	58.2
rpoB-trnC-GCA_p5	ATTGGATCCCCACCTACACA	ATCAAGCGTCTGTTCCTACG	50.0	50.0	58.0	57.7	1906-1921 bp	0.5	58.0