Hotspot

ycf1

SSC · 126638-131974 · 5337 bp

Back to Hesperaloe

Pi 0.00337

MarkerSeek score 54.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5337
variable_sites: 18
pi: 0.00337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAACCTCTTATTGCTTGAGT	ACAAACGTGCATTCTCCACT	35.0	45.0	52.4	58.0	251 bp	1.0	41.0
ycf1_p2	ACATTAAACCTCTTATTGCTTGAGT	ACAAACGTGCATTCTCCACT	32.0	45.0	57.0	58.0	256 bp	1.0	40.9
ycf1_p3	TGGGTTACTAACATTAAACCTCT	ACAAACGTGCATTCTCCACT	34.8	45.0	55.0	58.0	266 bp	1.0	40.9
ycf1_p4	TGGGTTACTAACATTAAACCTCTT	ACAAACGTGCATTCTCCACT	33.3	45.0	55.7	58.0	266 bp	1.0	40.9
ycf1_p5	TTGGGTTACTAACATTAAACCTCT	ACAAACGTGCATTCTCCACT	33.3	45.0	55.7	58.0	267 bp	1.0	40.9