Hotspot

trnR-UCU

LSC · 10540-10611 · 72 bp

Back to Hesperaloe

Pi 0.0139
MarkerSeek score 60.50
Primer available yes
Label trnR-UCU

Feature Metadata

label_name
trnR-UCU
feature_type
tRNA
region
LSC
length_bp
72
variable_sites
1
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU_p1 TCCATTCGGATATGCATGCT AGGGTTTCTTGATATCGCCA 45.0 45.0 57.1 56.6 224-226 bp 1.0 63.0
trnR-UCU_p2 TCCATTCGGATATGCATGCT AAGGGTTTCTTGATATCGCCA 45.0 42.9 57.1 57.4 225-227 bp 1.0 61.0
trnR-UCU_p3 TCCATTCGGATATGCATGCTT AGGGTTTCTTGATATCGCCA 42.9 45.0 57.9 56.6 224-226 bp 1.0 60.7
trnR-UCU_p4 TCCATTCGGATATGCATGCT AGGGTTTCTTGATATCGCCAT 45.0 42.9 57.1 57.1 224-226 bp 1.0 59.2
trnR-UCU_p5 TCCATTCGGATATGCATGCTT AAGGGTTTCTTGATATCGCCA 42.9 42.9 57.9 57.4 225-227 bp 1.0 58.7