Hotspot

rpl22

LSC · 85677-86057 · 381 bp

Back to Hesperaloe

Pi 0.00787

MarkerSeek score 50.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 381
variable_sites: 3
pi: 0.00787
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	CATTCATTGGCGGGGGATAA	47.6	50.0	58.1	58.0	519 bp	1.0	71.9
rpl22_p2	CCAAGTCTGAAACCAAGTGGA	TTATCATTCATTGGCGGGGG	47.6	50.0	58.1	58.0	523 bp	1.0	71.9
rpl22_p3	CCAAGTCTGAAACCAAGTGGA	TTCATTGGCGGGGGATAAAC	47.6	50.0	58.1	58.2	517 bp	1.0	70.1
rpl22_p4	ACCAAGTCTGAAACCAAGTGG	CATTCATTGGCGGGGGATAA	47.6	50.0	58.3	58.0	520 bp	1.0	69.7
rpl22_p5	ACCAAGTCTGAAACCAAGTGG	TTATCATTCATTGGCGGGGG	47.6	50.0	58.3	58.0	524 bp	1.0	69.6