Hotspot

rpl16

LSC · 83390-84782 · 1393 bp

Back to Hesperaloe

Pi 0.00287

MarkerSeek score 49.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1393
variable_sites: 4
pi: 0.00287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCTCAATACCCCTTTTGTT	TCTGTTTTTCCGTTAAATCAAA	40.0	27.3	54.1	52.8	1524 bp	1.0	40.2
rpl16_p2	TCCTCAATACCCCTTTTGTT	TTCTGTTTTTCCGTTAAATCAA	40.0	27.3	54.1	52.8	1525 bp	1.0	40.2
rpl16_p3	TCCTCAATACCCCTTTTGTT	TTTCTGTTTTTCCGTTAAATCA	40.0	27.3	54.1	52.8	1526 bp	1.0	40.2
rpl16_p4	TCCTCAATACCCCTTTTGTTT	TCTGTTTTTCCGTTAAATCAAA	38.1	27.3	55.0	52.8	1524 bp	1.0	40.2
rpl16_p5	TCCTCAATACCCCTTTTGTTT	TTCTGTTTTTCCGTTAAATCAA	38.1	27.3	55.0	52.8	1525 bp	1.0	40.2