Hotspot

ndhF

SSC · 112800-115028 · 2229 bp

Back to Hesperaloe

Pi 0.00404

MarkerSeek score 45.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 9
pi: 0.00404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATCCGCCGAAGAAGACAAAG	ACCCAATCAATCAGTTCCGC	50.0	50.0	58.3	58.5	2928-2952 bp	1.0	74.6
ndhF_p2	CCGCCGAAGAAGACAAAGAT	ACCCAATCAATCAGTTCCGC	50.0	50.0	58.3	58.5	2926-2950 bp	1.0	74.6
ndhF_p3	ATCCGCCGAAGAAGACAAAG	CCCAATCAATCAGTTCCGCA	50.0	50.0	58.3	58.5	2927-2951 bp	1.0	74.6
ndhF_p4	CCGCCGAAGAAGACAAAGAT	CCCAATCAATCAGTTCCGCA	50.0	50.0	58.3	58.5	2925-2949 bp	1.0	74.6
ndhF_p5	GATCCGCCGAAGAAGACAAA	ACCCAATCAATCAGTTCCGC	50.0	50.0	58.3	58.5	2929-2953 bp	1.0	74.5