Hotspot

ndhD

SSC · 118182-119684 · 1503 bp

Back to Hesperaloe

Pi 0.00466

MarkerSeek score 47.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 7
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCAAGGTACAAATTCGGCAC	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.0	58.0	2061-2072 bp	1.0	80.0
ndhD_p2	TTGGCAAGATCGAATACGGG	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.1	58.0	2350-2361 bp	1.0	79.7
ndhD_p3	GCAAGGTACAAATTCGGCAC	TGTTGGCCAATGTGAAGTCA	50.0	45.0	58.0	57.9	2461-2471 bp	1.0	79.7
ndhD_p4	TGGGACCCCAAAGAAACTTG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	2141-2152 bp	1.0	79.5
ndhD_p5	TTGGCAAGATCGAATACGGG	TGTTGGCCAATGTGAAGTCA	50.0	45.0	58.1	57.9	2750-2760 bp	1.0	79.4