Hotspot

rpl32-trnL-UAG

SSC · 116153-116838 · 686 bp

Back to Hesperaloe

Pi 0.00444
MarkerSeek score 55.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
686
variable_sites
3
pi
0.00444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GTGCAACAAACAGCAGGTAA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.1 58.0 741-751 bp 1.0 74.4
rpl32-trnL-UAG_p2 GTGCAACAAACAGCAGGTAA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.1 58.0 742-752 bp 1.0 74.4
rpl32-trnL-UAG_p3 GTGCAACAAACAGCAGGTAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.1 57.9 751-761 bp 1.0 73.5
rpl32-trnL-UAG_p4 GTGCAACAAACAGCAGGTAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.1 57.8 750-760 bp 1.0 72.9
rpl32-trnL-UAG_p5 GTGCAACAAACAGCAGGTAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.1 57.8 749-759 bp 1.0 72.9