Hotspot

ycf4-cemA

LSC · 62165-63018 · 854 bp

Back to Hesperaloe

Pi 0

MarkerSeek score 61.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.9	57.8	869-959 bp	1.0	82.1
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.1	57.8	871-961 bp	1.0	81.8
ycf4-cemA_p3	TGCGCGTACCAATTGAAGTA	GGGAGGGAAGTCAATGCTTT	45.0	50.0	57.9	57.8	822-912 bp	1.0	81.6
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	GGGAGGGAAGTCAATGCTTT	50.0	50.0	58.1	57.8	824-914 bp	1.0	81.3
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.7	57.8	874-964 bp	1.0	80.1