Hotspot

atpB-rbcL

LSC · 56286-57086 · 801 bp

Back to Hesperaloe

Pi 0.00375

MarkerSeek score 60.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 3
pi: 0.00375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	909 bp	1.0	68.2
atpB-rbcL_p2	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.5	56.6	910 bp	1.0	65.3
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	57.9	57.2	909 bp	1.0	64.4
atpB-rbcL_p4	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	933 bp	1.0	63.9
atpB-rbcL_p5	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	872 bp	1.0	63.7