Hotspot

trnE-UUC-trnT-GGU

LSC · 32298-32780 · 483 bp

Back to Hesperaloe

Pi 0.0166

MarkerSeek score 65.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 8
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGAATCCCCGTTTTCTCCTT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	595-607 bp	1.0	80.1
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	624-636 bp	1.0	79.7
trnE-UUC-trnT-GGU_p3	CGAATCCCCGTTTTCTCCTT	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	574-586 bp	1.0	79.7
trnE-UUC-trnT-GGU_p4	GGAAGTCGAATCCCCGTTTT	GAACCGATGACTTACGCCTT	50.0	50.0	58.2	58.0	601-613 bp	1.0	79.5
trnE-UUC-trnT-GGU_p5	CGAATCCCCGTTTTCTCCTT	TGGCGTTACTCTACCACTGA	50.0	50.0	57.9	58.1	571-583 bp	1.0	79.5