Hotspot

ycf1

SSC · 125600-131176 · 5577 bp

Back to Herpetospermum

Pi 0.0281

MarkerSeek score 58.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 154
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTTATCATTACATAATTGAGTCCT	TGTTCACAACAGAAAAACTTTCT	26.9	30.4	54.0	55.4	181 bp	1.0	44.9
ycf1_p2	AGTTTTATCATTACATAATTGAGTCCT	TGTTCACAACAGAAAAACTTTCT	25.9	30.4	55.2	55.4	182 bp	1.0	44.8
ycf1_p3	AGTTTTATCATTACATAATTGAGTCC	TGTTCACAACAGAAAAACTTTCT	26.9	30.4	54.0	55.4	182 bp	1.0	44.8
ycf1_p4	GTTTTATCATTACATAATTGAGTCCT	TCTTTGTTCACAACAGAAAAACT	26.9	30.4	54.0	55.4	185 bp	1.0	44.8
ycf1_p5	AGTTTTATCATTACATAATTGAGTCCT	TCTTTGTTCACAACAGAAAAACT	25.9	30.4	55.2	55.4	186 bp	1.0	44.7